Fig. 8.

HDAC4 is not required for Gadd45a expression or muscle fiber atrophy during fasting. A: TA muscles of C57BL/6 mice were transfected with 20 μg p-miR-Control, 20 μg p-miR-HDAC4 #1, or 20 μg p-miR-HDAC4 #2, as indicated. Seven days later, mice were divided into two cohorts, fed and fasted. Fed mice continued to have ad libitum access to food; food was removed from fasted mice. Twenty-four hours later, TA muscles were harvested for qPCR analysis of Gadd45a mRNA. B: TA muscles of C57BL/6 mice were transfected with 20 μg p-miR-Control, 20 μg p-miR-HDAC4 #1, or 20 μg p-miR-HDAC4 #2. Seven days later, mice were fasted for 24 h, and then TA muscles were harvested for histological analysis. Data are fiber size distributions from ≥4 mice/condition. Mean fiber diameters ± SE in the presence of miR-Control, miR-HDAC4 #1, and miR-HDAC4 #2 were 33 ± 0, 33 ± 1, and 33 ± 2 μm, respectively. These differences were not statistically significant.