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Nitric oxide (NO)-mediated inhibition of TC uptake does not occur through C266. Cells were transfected with either WT or C266A Ntcp. Later (48 h), cells were treated with or without various doses of S-nitrosocysteine (CysNO, 0.0625–1 mM) for 30 min, and then relative TC uptake was determined. Data are expressed as means ± SE (n = 3). *Significantly different from WT Ntcp control values (P < 0.05). # Significantly different from C266A Ntcp control values (P < 0.05).
