Expression of transient receptor potential vanilloid (TRPV)5, TRPV6, calbindin D28k, and klotho in kidney from SD and GHS fed LCD, without or with 1,25D. Each sample's RNA content was normalized to the geometric mean of 4 markers (β-actin, rpl13a, YHAWAH, and sdh) and then expressed as relative to the level of that particular gene product in SD as a unitless ratio (means ± SE). *P < 0.05 compared with SD. +P < 0.05 compared with GHS. oP < 0.05 compared with SD+1,25D.