Fig. 2.

mTOR signaling is activated in the renal medulla of lithium-treated mice. Mice were fed a 0.2% LiCl diet for 2 wk. Top: Western blot analysis of protein homogenates from control and lithium-treated mice was probed for PCNA, p-rS6, p-4EBP, p-TSC2, and p-Akt. Total rS6, 4EBP, TSC2, and Akt were used as loading control for densitometry. Each lane represents a homogenate from an individual medulla. Bottom: densitometry results for top. Each phospho protein was normalized to total protein, n = 3 vs. 3. *Significant difference between control mice and lithium-treated mice (t-test, P < 0.05).