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Rapamycin reverses lithium-induced cell proliferation in medullary collecting ducts. Proliferating cell nuclear antigen (PCNA) immunoperoxidase labeling was performed in paraffin-embedded mouse kidney sections from control mice, lithium-treated mice, and lithium/rapamycin-treated mice. Representative photographs depict PCNA-positive cells in the renal medulla, demonstrating that rapamycin reverses lithium-induced proliferation in the initial mouse renal medulla (magnification ×200).
