Fig. 6.

Rapamycin inhibition did not restore AQP2 or UT-A1 expression. Mice were fed lithium (0.2%) or control diet for 4 wk and injected daily with rapamycin or placebo for 2 wk. Western blot analysis of protein homogenates from control, lithium-, lithium/rapamycin-, and rapamycin-treated mice was probed for AQP2, UT-A1, UT-A3, and p-GSK3α and β. Total GSK3β was used as loading controls for densitometry. A: representative blots. Each lane represents a homogenate from an individual mouse. B: densitometric analysis of results for AQP2, UT-A1, and p-GSK3β is expressed as means ± SE, n = 4 in control vs. 5 experimental groups. *Significant difference vs. control mice (t-test, P < 0.05).