Table 1.
Effect of lithium on gene expression in mouse renal medulla
| Gene | C vs. Li | C vs. Li-Rapa | C vs. Rapa | Li vs. Li-Rapa |
|---|---|---|---|---|
| AQP2 | 0.66 | 0.49* | 1.20 | 0.82 |
| COX-2 | 1.09 | 0.47* | 0.67 | 0.43* |
| Egr-1 | 5.46* | 1.27 | 0.93 | 0.23 |
| Cyclin D1 | 1.73* | 2.09 | 1.64* | 1.20 |
| c-Ret | 0.75 | 0.59 | 0.50 | 0.79 |
| c-Ret9 | 0.36* | 0.35* | 0.34* | 0.96 |
| c-Ret51 | 0.63 | 0.55 | 0.45 | 0.88 |
SYBR green real-time PCR assay was used to derive Ct values for calculation of mRNA abundance. Data shown are mean relative gene expression normalized to control group. AQP2, aquaporin-2; COX-2, cyclooxygenase-2; C, control; Li, lithium; Rapa, rapamycin.
*
Significant difference between groups (t-test, P < 0.05).