Fig. 5.

Altered antimicrobial peptide production in Irgm1 KO mice. Male WT and Irgm1 KO mice were given 3% DSS in drinking water (DSS) or maintained on standard drinking water (cont) for 7 days. Quantitative RT-PCR was used to measure transcript levels of selected molecules from several mouse antimicrobial peptide classes, including Lyz, Reg3γ, and Ang4 (A), representative α-defensin isoforms, including Defa3, Defa5, and Defa20 (B), and villin (Vil1, C), as an indicator of total epithelial cell mass. Values (means ± SE) are normalized to β-actin and expressed as fold change relative to the WT control group. Cohort sizes were as follows: n = 13 WT control, 11 KO control, 10 WT DSS, and 9 KO DSS. *P ≤ 0.05.