PCR analysis of Helicobacter isolates. The three Helicobacter isolates were H. pylori (lanes 1, 4, 7, and 10) (50), H. hepaticus (lanes 2, 5, 8, and 11) (15), and H. hepaticus (used in the current study, lanes 3, 6, 9, and 12). The PCR primers used were as follows: 16s universal primers (27F and 1391R) (56), lanes 1–3; Helicobacter group specific (274F and 1216R) (50), lanes 4–6; 16s H. hepaticus specific (591F and 979R) (2) lanes 7–9; and p25 H. hepaticus specific (p25F and p25R) (15), lanes 10–12. Phi-X174/HaeIII was used as size standard marker.