Fig. 2.

Sodium bisulfite modification and genomic sequencing of the αENaC promoter reveals cytosine methylation within and near the +222/+229 Sp1 site. mIMCD3 cells were treated with vehicle or 0.5 μg/ml 5-Aza-CdR for 24 h. Genomic DNA was harvested and exposed to sodium bisulfite to modify methylated cytosine to uracil. DNA sequencing was then performed. A representative (n = 3) sequencing graph of the +238/+207 region is shown. Arrows indicate modified and unmodified cytosines. The red boxes outline the +222/+229 Sp1 element.