Fig. 3.

The DNA methylation inhibitor 5-Aza-CdR augments αENaC mRNA expression and αENaC promoter activity in mIMCD3 cells. A: mIMCD3 cells were treated with vehicle or 0.5 μg/ml 5-Aza-CdR, together with vehicle (−Aldo) or 1 μM aldosterone (+Aldo) for 24 h. A: RNA was then prepared for quantitative (q)RT-PCR to measure αENaC mRNA levels. n = 6. *P < 0.05 vs. vehicle (−Aldo). B: mIMCD3 cells stably transfected with pcDNA3.1-Zeo-1.3αENaC-Luc were treated as described in A, and cell lysates were then prepared for luciferase activity measurements, which were normalized to cell protein content. n = 6. *P < 0.05 vs. vehicle (−Aldo).