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Cytosine methylation inhibits Sp1 binding to the αENaC promoter. mIMCD3 cells were treated in the presence of vehicle (v..) or the DNA methylation inhibitor 5-Aza-CdR (5..) as described in Fig. 3. Chromatin immunoprecipitation (ChIP)/qPCR analysis of the R3 subregion of the αENaC gene using anti-Sp1 antibodies (or nonimmune IgG as a negative control) was performed (n = 3). Error bars indicate ±SEs. *P < 0.05 vs. vehicle.
