Fig. 6.

Methyl-CpG-binding domain protein (MBD)4 regulates αENaC gene expression in mIMCD3 cells. A: siRNA knockdown of MBD4 induces basal αENaC gene expression in mIMCD3 cells. mIMCD3 cells were transiently transfected with control siRNA or with MBD4-specific siRNA. Cells were then treated with vehicle or Aldo, and αENaC mRNA, which was normalized to β-actin mRNA, was measured. n = 6. *P < 0.05 vs. vehicle-treated control siRNA. B: mIMCD3 cells stably transfected with pcDNA3.1-Zeo-1.3αENaC-Luc were transiently transfected with control vector or a MBD4 expression plasmid. Cells were then treated with vehicle or aldosterone for 24 h, and luciferase activity, which was normalized to cell protein content, was measured. n = 6. *P < 0.05 vs. Aldo-treated control siRNA.