Fig. 6.

NF-κB and calcium handling. A: calcium transients during simulated ischemia-reperfusion. Myocytes were field stimulated (cycle length = 1 s), and transients were recorded. Representative calcium transients during control (black), after 5 min of ischemia followed by 5 min of reperfusion (blue) (n = 7), are shown. Western blot analysis (B) and densitometry (C) of whole mouse hearts for sarco(endo)plasmic reticulum Ca²⁺-ATPase (SERCA2a), phospholamban (PLN), and phosphorylated PLN-Ser¹⁶ 6 h after in vivo regional ischemia-reperfusion (n = 4 to 5). *P < 0.05 compared with WT.