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Effect of pH, temperature, ATP, and KCl on cooperativity (n50; top) and Hb-O2 affinity (P50; bottom) for T. scripta HbA (A) and HbD (B). Conditions: stripped Hb (0.2 mM heme) in the absence (●) and the presence of 0.15 mM ATP (▲) or 0.1 M KCl (△) at 20°C and stripped at 10°C (○). All samples were run in triplicate in 0.1 M HEPES buffer. Data points with error bars indicate means ± SE.
