Table 1.
Oxygenation properties (0.1 M HEPES, 20°C, pH 7.4) of T. scripta HbA and HbD, including O2 affinity, cooperativity coefficient, and Bohr factor
|
P50 (mmHg) |
n50 |
φ |
||||
|---|---|---|---|---|---|---|
| HbA | HbD | HbA | HbD | HbA | HbD | |
| Stripped | 3.00 ± 0.07 | 1.92 ± 0.04 | 2.13 ± 0.08 | 1.95 ± 0.10 | −0.34 ± 0.02 | −0.03 ± 0.02 |
| 0.15 mM ATP | 14.90 ± 0.56 | 9.36 ± 0.60 | 2.73 ± 0.08 | 2.70 ± 0.07 | −0.68 ± 0.02 | −0.65 ± 0.06 |
| 0.1 M Cl− | 5.87 ± 0.51 | 3.09 ± 0.10 | 2.46 ± 0.29 | 2.51 ± 0.07 | −0.40 ± 0.05 | −0.12 ± 0.03 |
Values are expressed as means ± SE. HbA, hemoglobin isoform A; HbD, hemoglobin isoform D; P50, O2 affinity; n50, cooperativity coefficient; φ, Bohr factor. φ = ΔlogP50/ΔpH, pH range 6.9–8.0.