Table 2.
Mean 24-h rhythm analysis for core body temperature and activity
| LD | DD |
|||
|---|---|---|---|---|
| 13d | AdLib1 13 days | FR 10 days | AdLib2 10 days | |
| Tb °C | ||||
| Period, h | ||||
| B-SAP | 24.0 ± 0.0 | 24.4 ± 0.0* | 24.4 ± 0.2 | 24.8 ± 0.2a |
| Lep-SAP | 24.1 ± 0.0 | 24.3 ± 0.3 | 24.3 ± 0.3a | 24.6 ± 0.3 |
| Amplitude (robustness) | ||||
| B-SAP | 487.0 ± 34.5 | 305.9 ± 34.8a | 219.4 ± 41.7a | 181.7 ± 24.3a |
| Lep-SAP | 248.7 ± 34.4* | 118.6 ± 23.5*a | 207.4 ± 21.3 | 62.9 ± 9.5*a |
| % Rhythmic | ||||
| B-SAP | 100 | 100 | 100 | 100 |
| Lep-SAP | 100 | 100 | 100 | 67 |
| Activity | ||||
| Period, h | ||||
| B-SAP | 24.0 ± 0.0 | 24.3 ± 0.0a | 24.4 ± 0.1a | 24.1 ± 0.1 |
| Lep-SAP | 24.0 ± 0.1 | No rhythm | 24.0 ± 0.1* | 23.9 |
| Amplitude (robustness) | ||||
| B-SAP | 117.3 ± 13.6 | 40.3 ± 6.0a | 25.6 ± 8.1a | 32.3 ± 7.5a |
| Lep-SAP | 22.2 ± 2.8* | No rhythm | 24.7 ± 2.2 | 15* |
| % Rhythmic | ||||
| B-SAP | 100 | 80 | 100 | 30 |
| Lep-SAP | 50 | 0 | 100 | 8 |
Amplitude (robustness) refers to the consistency of the rhythm across days. For the first 13 days the rats are in 12:12-h light:dark (LD) and, thereafter, in dark:dark (DD). For the first 13 days of DD, all rats were ad libitum fed (AdLib1). For the next 10 days, food availability was restricted to food availability for 4 hours per day from 9 AM until 1 PM (FR). This was followed by a 3-day fast (insufficient time for rhythm analysis). Then all rats were returned to ad libitum feeding (AdLib2).
*
Asterisk is significant difference between groups (P < 0.05; “a” is significant difference within groups compared to LD (P < 0.05).