Fig. 1.

Soluble guanylate cyclase (sGC) isoform protein expression is decreased in sGC-α1 knockout (KO) mouse pup lung. cGMP signaling enzyme protein expression in lungs of 10-day-old sGC-α1 KO and wild-type (WT) mouse pups was determined using immunoblotting. A: representative protein blots of 50 μg of soluble lung proteins from 2 mouse pups in each group that were reacted with indicated antibodies. α-Tubulin immunoreactivity was assessed to demonstrate that equal amounts of lung proteins were analyzed. PKGI, cGMP-dependent protein kinase. B: band densities of indicated protein immunoreactivity normalized to the band density of α-tubulin immunoreactivity. N = 5–6 mice per group. *P < 0.05 for difference between genotypes.