Fig. 7.

Diminished sGC activity decreases smooth muscle myosin heavy chain (SMMHC) expression in the 70% oxygen-injured peripheral mouse pup lung. SMMHC immunoreactivity was determined using mouse pup lung sections using a specific polyclonal antibody and immunohistochemistry (depicted in red); cells were counterstained with hematoxylin. Whereas the peripheral lungs from P13 air- and 70% O₂-exposed WT and air-treated sGC-α1 KO pups exhibited SMMHC immunoreactivity in cells in the wall of peripheral lung arteries (arrow) and in alveolar septae (arrow head), SMMHC expression was decreased in 70% O₂-treated sGC-α1 KO pups. Representative images of 3 separate experiments are shown. Scale bar = 20 μm.