Fig. 1.
Stimulation of mitochondrial ATP-sensitive K+ channel (mitoKATP) with diazoxide induces production of mitochondrial and cytoplasmic superoxide (O2·−). A: mitochondrial O2·− was measured by MitoSOX (2 μM) and confocal microscopy in human aortic endothelial cells (HAECs) following stimulation with angiotensin II (ANG II; 200 nM) or diazoxide (100 nM). PKCε peptide antagonist H-Glu-Ala-Val-Ser-Leu-Lys-Pro-Thr-OH (EAVSLKPT; 1 μM) was added before MitoSOX application. B: cytoplasmic O2− was measured by dihydroethidium (DHE; 10 μM) and confocal microscopy in HAECs stimulated by ANG II or diazoxide. PKCε peptide antagonist EAVSLKPT (1 μM) was added before DHE application. Inh, inhibitor. Data represent typical Z-stack of fluorescent images.