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Redox sensitivity of diazoxide-induced O2·− production. Mitochondrial O2·− was measured by MitoSOX (A); cytoplasmic O2·− was detected by DHE (B), and mitochondrial potential was analyzed by TMRM fluorescence (C). HAECs were treated with 1 nM mitoEbselen 15 min before stimulation with diazoxide (100 nM, 10 min).
