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Production of mitochondrial O2·− was attenuated by inhibition of reverse electron transfer (RET) with rotenone, malonate, or with PKCε peptide antagonist EAVSLKPT. Mitochondrial O2·− was measured using fluorescent probe MitoSOX and confocal microscopy in unstimulated cells (basal; A) and HAECs stimulated with diazoxide (100 nM, 10 min; B) and then treatment with rotenone, malonate, or PKCε peptide antagonist EAVSLKPT before application of MitoSOX.
