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Diazoxide-induced cytoplasmic O2·− is attenuated by inhibition of RET with rotenone, malate, or PKCε peptide antagonist EAVSLKPT. Cytoplasmic O2·− was measured by DHE (10 μM) in HAECs treated with diazoxide (100 nM, 10 min), malonate (0.1 mM), rotenone (1 μM), PKCε peptide antagonist EAVSLKPT (1 μM), or Nox2 inhibitor peptide gp91ds or cSrc inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolopyrimidine (PP2).
