Fig. 4.

Expression of mammalian TTP mRNA in chicken embryonic fibroblasts (CEF). CEF were either mock-transfected (Construct -), or transfected with plasmids containing the genomic mouse TTP (gmTTP, lanes 3 and 4), human genomic TTP (ghTTP, lanes 6 and 7), or genomic bovine TTP (gbTTP, lanes 10 and 11) DNA. Twenty four hours later, the cells were serum-deprived for a further 24 h, and then 10% FBS was added to groups of dishes (FBS +) for 60 min. Each lane was loaded with 20 μg of total cellular RNA, and the Northern blot was hybridized with the ³²P-labeled mouse TTP cDNA probe (lanes 1–4), the human TTP cDNA probe (lanes 5–7), or the bovine TTP cDNA probe (lanes 8–11).