Fig. 5.
TTP and Zfp36l1 mRNA expression in mouse and chicken macrophages. Chicken HD11 and mouse Raw264.7 cells were serum-deprived for 24 h and then treated with LPS (1 μg/ml) for the indicated times. Each lane was loaded with 15 μg (lanes 1–6) or 5 μg (lanes 7–12) of total cellular RNA, and the Northern blot was hybridized with the 32P-labeled mouse TTP cDNA probe (top), or the chicken Zfp36l1 cDNA probe (bottom). Zfp36l1 mRNA expresses at low levels in Raw264.7 cells and is barely detectable, even using the rat Zfp36l1 cDNA probe (not shown).