Fig. 7.

Chicken cell RNA samples for mRNASeq. After serum deprivation for 24 h, CEF or HD11 cells were treated for 60 min with FBS (10%) or LPS (1 μg/ml), respectively. Each gel lane was loaded with 10 μg of total cellular RNA, and the Northern blot was hybridized with the ³²P-labeled chicken Zfp36l1 cDNA probe (top) or the chicken Zfp36l2 cDNA probe (bottom). The double arrows in the upper panel indicate the two bands of Zfp36l1 mRNA, and the single arrow in the lower panel indicates the single species of Zfp36l2 mRNA. The lower bands apparent in the HD11 cells represent cross-hybridization of the Zfp36l2 probe with Zfp36l1 mRNA.