Fig. 2.

Transient receptor potential vanilloid (TRPV1) expression in subfornical organ (SFO). Top: immunohistochemical demonstration of TRPV1 expression in SFO. Note the prominent expression in thick astrocytic processes and the lack of neuronal perikaryal staining. Bottom: significant reduction in TRPV1 staining in SFO 28 days postintracerebroventricular injection of AAV1/2-shRNA-TRPV1 (GCG CAU CUU CUA CUU CAA C) demonstrating antibody specificity. A COOH-terminal directed antibody (Cat. no. GP14100; Neuromics, Edina, MN) was used for the immunohistochemistry.