Fig. 2.

Dissected cortex and whole liver were harvested from wild-type (WT) 129/sv mice euthanized at midnight. Nuclear extracts were collected and coimmunoprecipitations (Co-ip) were performed using anti-Per1 antibody with respective positive and negative controls. Positive control consisted of protein input. Negative controls consisted of replacing anti-Per1 resin with inactive cross-linked resin. Data are representative of three independent experiments. Western blot analysis was performed using anti-Per1, anti-Cry2, anti-Clock, anti-Bmal1, and anti-Per2 antibodies.