Fig. 3.
αENaC and Fxyd5 are Clock targets, whereas ET-1 is Clock-independent. mpkCCDc14 cells were treated with nontarget, Per1, or Clock siRNA or a combination of Per1 and Clock short interfering RNA (siRNA) for 48 h. Quantitative real-time polymerase chain reaction (QPCR) was used to evaluate changes in Per1 (A), Clock (B), αENaC (C), Fxyd5 (D), or ET-1 (E) gene expression in Per1, Clock, or Clock/Per1 siRNA treated cells vs. nontarget siRNA control. *P < 0.05, **P < 0.01, ***P < 0.001; n = 4. Values are means ± SE.