Fig. 4.
Per1 knockdown results in upregulation of Cry2 in vitro in mpkCCDc14 cells. Lentiviral particles either containing nontarget or Per1 shRNA were transfected into 40,000 mpkCCDc14 cells; puromycin- and GFP-selected; and 10 individual clones were randomly selected, separated, and grown to confluence. Western blot analysis was performed using anti-Per1, anti-Bmal1, anti-Clock, anti-Cry2 antibodies. Anti-β-actin was used as a loading control. Densitometry analysis was used to quantitate the level of Per1 and Cry2 in (A). *P < 0.05 relative to nontarget short hairpin RNA (shRNA); n = 3. Values are means ± SE.