Fig. 7.

Reduced Per1 levels in vivo results in decreased levels of PPARα and DEC1. Whole livers were harvested from WT 129/sv and Per1 het mice euthanized at midnight; nuclear extracts and RNA were collected. A and B: changes in PPARα and DEC1 mRNA were determined by QPCR. *P < 0.05; n = 4. Western blot analysis was performed using anti-PPARα, or anti-DEC1 to evaluate changes in PPARα (C) and DEC1 (D) protein expression with β-actin as a loading control. Data are representative of four independent experiments. Values are means ± SE.