Fig. 9.

Per1 knockdown results in upregulation of Cry2 in vitro in AML12 cells. A: nuclear and cytosolic extracts were collected from AML12 cells treated with 10 μM casein kinase 1δ/ε inhibitor PF670462 for 72 h. Western blot analysis was performed using anti-Cry2 or anti-β-actin antibodies as a loading control. Data are representative of three independent experiments. B: densitometry analysis was used to quantitate the level of Cry2 in (A). *P < 0.05 relative to vehicle, †P < 0.5 relative to cytosolic; n = 3. Values are means ± SE.