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pSTAT3 level after leptin induction in hypothalamus and cortex. A: in hypothalamus, ip injection. B: in hypothalamus, icv injection. C: in cortex, ip injection. D: in cortex, icv injection. Leptin dose and induction time: ip: 3 mg/kg, 45 min; icv: 2.5 μg/mouse, 30 min. *P < 0.05, ***P < 0.001, ****P < 0.0001.
