. 2013 Jul 18;115(7):1065–1074. doi: 10.1152/japplphysiol.00611.2013

Table 2.

Differentially regulated genes involved in the integrin-linked kinase signaling

				Fold Change
Type(s)	Symbol	Gene Name	Member of/Molecule	d3	d5	d7
Binding protein	IRS1	Insulin receptor substrate 1	IRS	0.50	0.77	0.96
	SH2B2	SH2B adaptor protein 2	IRS	2.25	2.03	1.77
	TMSB10/TMSB4X	Thymosin β4, X-linked	TMSB4	6.41	9.05	8.47
Cyclin	CCND1	Cyclin D1	CCND1	3.99	3.35	3.13
Cytokine	TNF	Tumor necrosis factor	TNF	2.30	1.78	1.34
Cytoskeleton protein	ACTG1	Actin, γ1	F-Actin	2.11	2.16	2.19
	ACTG2	Actin, γ2, smooth muscle, enteric	G-Actin/F-Actin	0.93	2.05	2.82
	ACTN4	Actinin, α4	α-Actinin	2.03	2.14	2.32
	FBLIM1	Filamin binding LIM protein 1	Filanin	2.40	2.91	2.91
	FLNA	Filamin A, α	Filanin	2.45	2.92	3.52
	FLNB	Filamin B, β	Filanin	2.50	2.94	3.17
	MYH9	Myosin, heavy chain 9, nonmuscle	Myosin	3.08	3.25	3.44
	PARVA	Parvin, α	PARVA	2.26	2.98	3.19
	VIM	Vimentin	Vimentin	2.15	2.16	2.20
Enzyme	FNBP1	Formin binding protein 1	Ras homolog	2.02	2.20	2.43
	PTGS2	Prostaglandin-endoperoxide synthase 2	PTGS2	6.92	8.21	8.67
	RHOB	Ras homolog family member B	Ras homolog	1.64	1.98	2.19
	RHOC	Ras homolog family member C	Ras homolog	2.90	3.05	2.72
	RHOU	Ras homolog family member U	Ras homolog	0.72	0.55	0.46
	RND3	Rho family GTPase 3	Ras homolog	1.87	2.26	2.64
Extracellular matrix protein	FN1	Fibronectin 1	Fibronectin 1	3.10	3.91	4.32
Growth factor	PDGFC	Platelet-derived growth factor C	VEGF	2.98	4.72	5.27
	VEGFA	Vascular endothelial growth factor A	VEGF	0.57	0.39	0.28
	VEGFB	Vascular endothelial growth factor B	VEGF	0.42	0.38	0.32
Kinase	AKT1	v-Akt murine thymoma viral oncogene homolog 1	AKT	1.90	2.11	2.17
	ILK	Integrin-linked kinase	ILK	1.92	2.28	2.53
	MAP2K6	Mitogen-activated protein kinase kinase 6	MAP2K6	0.18	0.24	0.26
	MAPK12	Mitogen-activated protein kinase 12	JNK	0.60	0.58	0.49
	PIK3C2A	Phosphoinositide 3-kinase, class 2, α-polypeptide	PI3K	1.56	1.87	2.23
	PIK3R3	Phosphoinositide-3-kinase, regulatory subunit 3 (γ)	PI3K	0.93	1.82	2.54
	RPS6KA5	Ribosomal protein S6 kinase, 90 kDa, polypeptide 5	MSK1/2	0.42	0.49	0.57
Peptidase	CASP3	Caspase 3, apoptosis-related cysteine peptidase	CASP3	2.42	2.85	2.95
Phosphatase	PPAP2B	Phosphatidic acid phosphatase type 2B	PPAP2B	2.35	2.60	2.57
	PPM1J	Protein phosphatase, Mg²⁺/Mn²⁺ dependent, 1J	PP2A	0.44	0.41	0.33
	PPM1L	Protein phosphatase, Mg²⁺/Mn²⁺ dependent, 1L	PP2A	0.23	0.24	0.26
	PPP2R1B	Protein phosphatase 2, regulatory subunit A, β	PP2A	2.38	2.27	2.28
Sarcomeric protein	ACTC1	Actin, α, cardiac muscle 1	G-Actin/F-Actin	1.68	5.39	6.47
	ACTA2	Actin, α2, smooth muscle, aorta	G-Actin/F-Actin	1.04	2.02	2.20
	MYH11	Myosin, heavy chain 11, smooth muscle	Myosin	0.38	0.43	0.49
	MYH4	Myosin, heavy chain 4, skeletal muscle	Myosin	0.68	0.56	0.50
	MYH7	Myosin, heavy chain 7, cardiac muscle, β	Myosin	0.28	0.39	0.57
	MYH8	Myosin, heavy chain 8, skeletal muscle, perinatal	Myosin	0.93	3.63	6.09
	MYL2	Myosin, light chain 2, regulatory, cardiac, slow	Myosin	0.24	0.32	0.18
	MYL3	Myosin, light chain 3, alkali; ventricular, skeletal, slow	Myosin	0.14	0.11	0.10
	MYL4	Myosin, light chain 4, alkali; atrial, embryonic	Myosin	2.31	5.79	6.39
Transcription regulator	HIF1A	Hypoxia inducible factor 1, α-subunit	HIF1A	3.20	3.55	3.79
	MYC	v-Myc myelocytomatosis viral oncogene homolog	MYC	8.43	5.93	4.89
	SNAI1	Snail homolog 1	SNAI1	2.96	2.47	1.92
	SNAI2	Snail homolog 2	SNAI2	2.15	4.36	4.54
Transmembrane receptor	TNFRSF1A	Tumor necrosis factor receptor superfamily, member 1A	TNFR	2.69	2.46	2.21

The nos. represent the fold change of gene expression after d3, d5, and d7 of mechanical overload compared with control non-overloaded muscles. Italicized numbers mean that the fold change in gene expression is either lower than 50% decrease or lower than 2-fold increase. The selected genes were differentially expressed (twofold increase or 50% decrease) at least one time between d3 and d7.