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Schemes of wild-type TEV (TEV-wt) and recombinant TEV-Ros1 and TEV-eGFP. Lines represent the viral 5' and 3' untranslated regions (5'UTR and 3'UTR) and boxes represent the viral cistrons P1, HC-Pro, P3, P3N-PIPO, 6K1, CI, 6K2, VPg, NIaPro, NIb and CP, as indicated. The red and green boxes represent Ros1 and eGFP markers as indicated. Sequences of NIaPro cleavage sites flanking the markers in TEV-Ros1 and TEV-eGFP are given. Arrowheads point the exact cleavage position of the protease.
