Table 2.

NLSs identified on Herpesviral DNA polymerase subunits and nuclear import molecular mechanisms. Pol, DNA polymerase catalytic subunit; PAP, DNA polymerase processivity factor; hNLS, hydrophobic NLS; cNLS, classical NLS. The single letter amino acid code is used; hydrophobic residues in hNLS are underlined, whereas basic residues in cNLSs are in boldface. s indicates the ability of the NLS to function when the subunit is uncomplexed; h indicates the ability of the signal to function once the holoenzyme is assembled. In the column “Ran” the sensitivity of nuclear transport to overexpression of the transdominant negative RanQ69L is reported; in the column “ATP”, sensitivity to depletion of intracellular ATP levels; X, sensitivity; ?, unknown.

Virus	Protein	Name	NLS type	Sequence	IMPs	Ran	ATP	Nuclear import	Notes	Reference
HSV-1	pol	UL30	hNLS	RRMLHR-1129	?	?	?	s	overlapping PAP binding domain	[56]
			cNLS bip	PAKRPRETPSPADPPGGASKPRK-1136	a/β	X	X	s + h		[53]
	PAP	UL42	cNLS bip	PTTKRGRSGGEDARADALKKPK-413	a/β	X	X	s + h		[55]
HCMV	pol	UL54	hNLS	PRRLHL	?	?	?	s	overlapping PAP binding domain	[56]
			cNLS	PAKKRAR-1159	a/β	X	X	s + h		[54]
	PAP	UL44	cNLS	PNTKKQK-431	a/β	X	X	s + h	regulated by phosphorylation	[52]
HHV-7	pol	U38	/	/	/	/	/	/
	PAP	U27	cNLS	PNSKRQR-361	?	X	X	s		[57]
KHSV-1	pol	pol-8	no NLS					h	requires PF-8 for import	[59]
	PAP	PF-8	cNLS	KRPHKRRSD-377	?	?	?	s + h	mediates translocation of holoenzyme	[59]
EBV	pol	BALF1	no NLS					h	requires BMRF1 for import	[60]
	PAP	BMRF1	cNLS	KHPKK-396	?	?	?	s + h	mediates translocation of holoenzyme	[58]