Skip to main content
. 2013 Sep 17;109(8):2248–2258. doi: 10.1038/bjc.2013.550

Figure 5.

Figure 5

PD173074 inhibited cancer cell invasion and migration. (A) A decrease in invasive ability measured using the Matrigel invasion assay in HOC313 cells upon treatment with 15 nM PD173074 after 20 h incubation. *P<0.01; **P<0.05. Twenty thousand cells in 100 μl Dulbecco's modified Eagle's medium (DMEM) with or without PD173074 were plated in Matrigel-coated inserts with an 8 μm pore size. Following culture in 500 μl DMEM for 20 h, cells on the lower surface of the insert membrane were fixed, stained with haematoxylin and counted. Similar results were obtained in three independent experiments. Data represent the means±s.d. (n=3). (B) A decrease in migration ability was measured using the migration wound healing assay in HOC313 cells upon treatment with 15 nM PD173074 for 24 or 48 h. Two million cells were plated 24 h before PD173074 treatment and were then scraped with a plastic tip. Photomicrographs were taken at 24 and 48 h after wound formation.