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. 2013 Sep 17;109(8):2248–2258. doi: 10.1038/bjc.2013.550

Figure 6.

Figure 6

PD173074 induced MET. (A) The expression of EMT master genes, MMPs and major epithelial markers was examined by reverse transcription–polymerase chain reaction (RT–PCR) in HOC313 cells upon treatment without or with 15 nM PD173074B after 1, 2, 3, 4, 5 and 6 days. GAPDH expression was used as a positive control (Cont). (B) The expression of E-cadherin and Snail-1 was analysed by western blotting upon treatment without or with 15 nM PD173074B for 1, 2, 3, 4, 5 and 6 days. Anti-β-actin was used as a loading control. (C) Cell morphological changes. Upper panel, cell morphology was analysed by fluorescent microscopy according to the immunolocalisation of F-actin at day 5 with or without PD173074 treatment. (D) Activator protein-1 (AP-1) binding sites in the promoter of EMT master genes. (E) β-Catenin expression and adherent junctions in HOC313 cells were examined by immunofluorescent staining at day 5 after PD173074 treatment (15 nM). DAPI, 4′,6-diamidino-2-phenylindole.