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. 2013 Sep 17;109(8):2155–2166. doi: 10.1038/bjc.2013.577

Figure 3.

Figure 3

Direct recognition of EGFR expressing HNSCC by EGFR875–889 reactive CD4 T-cell clones. (A) EGFR875–889 reactive CD4 T-cell clones were tested for their capacity to recognise antigen directly on EGFR-positive HLA-DR matched or mismatched HNSCC cells by IFN-γ production. The HLA-DR-negative cell line Jurkat was used as negative control. HLA-DR restriction of these responses was demonstrated by blocking tumour recognition with anti-HLA-DR mAb L243 (10 μg ml−1). Columns without bars had s.d. <10% the values of the mean. Results are representative of three separate experiments. (B) EGFR875–889-reactive CD4 T-cell clones were evaluated for their capacity to kill EGFR-positive HLA-DR-matched HNSCC cells. HLA-DR unmatched cell lines were used as negative controls. Points, mean of triplicate determinations, bars s.d. Points without bars had s.d. <10% the value of the mean. Results are representative of three separate experiments.