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A CuII binding titration of NKB monitored by circular dichroism. (A) Titration of NKB (77.5 μM, 30 mM SDS, 10 mM nEM, pH 7.6) with CuIICl2 monitored between 400 and 700 nm. (B) Titration of NKB (77.5 μM, 30 mM SDS, 10 mM nEM, pH 8.7) with CuIICl2 monitored between 400 and 700 nm. (C) Molar ellipticity at 490 and 570 nm at pH 7.6 (■) and pH 8.7 (▼) plotted as a function of CuII equivalents highlights saturation at 0.5 equiv of CuII.
