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NiII binding titrations of NKB monitored by UV/vis spectroscopy. (A) NKB (108.5 μM) prepared in 30 mM SDS, 10 mM n-EM pH 8.9 was titrated with NiCl2 and the absorbance between 300 and 600 nm was monitored. The inset shows the absorbance at 420 nm plotted as a function of copper equivalents added ([NiII]/[NKB]).
