Table 4.
Positive Cases (FTA card) for EZH2 and CD79B Mutations, With List of Additional Samples Tested
| Case | Type of Specimen | Final Diagnosis | Relation | Location | Mutation | Direct Sequencinga |
|---|---|---|---|---|---|---|
| EZH2 | ||||||
| 1 | FTA card | DLBCL | LN right iliac | Y646S | C | |
| 1.1 | Smear | FL I-II | Previous | Retroperitoneal mass | Y646S | C |
| 3b | FTA card | U-DLBCL/BL (DHL) | LN left neck supraclavicular | Y646S | C | |
| 12 | FTA card | FL I-II | LN right supraclavicular | Neg | NT | |
| 12.1 | Smear | FL III | Previous | LN left inguinal | Y646F | C |
| 12.3 | Frozen | DLBCL (60%)/FL IIIA-IIIB (40%) | Previous | LN left inguinal | Y646F | C |
| 21b | FTA card | FL I-II | LN left neck | Y646S | C | |
| 25 | FTA card | DLBCL | Retroperitoneal mass | Neg | NT | |
| 25.2 | Smear | FL I-II | Previous | LN right inguinal | Y646F | C |
| 27b | FTA card | DLBCL | Intra-abdominal mass | Y646F | C | |
| 48 | FTA card | LBCL | LN left upper neck | Y646C | C | |
| 48.1 | Frozen/FFPE | FL IIIA | Subsequent | LN left cervical | Y646C | C |
| 58b | FTA card | LBCL | LN mesenteric | Y646F | C | |
| 62 | FTA card | FL I-II | LN left supraclavicular | Neg | NT | |
| 62.1 | Smear | FL I | Previous | LN right axila | Neg | NT |
| 62.2 | Frozen | FL II | Previous | LN right axila | Neg | NT |
| 62.3 | Smear | FL | Previous | LN right axila | Neg | NT |
| 62.5 | Smear | FL I | Previous | LN right supraclavicular | Neg | NT |
| 62.6 | Smear | FL I-II | Concurrent | LN left neck level II | Y646F | C |
| 70 | FTA card | FL I-II | LN left supraclavicular | Y646N | C | |
| 70.1 | Frozen | FL II-IIIA | Previous | LN right supraclavicular | Y646N | C |
| 76 | FTA card | FL I-II | LN right posterior cervical | Y646N | C | |
| 76.1 | Smear | Small B-Cell NHL | Previous | LN right submandibular | Y646N | C |
| CD79B | ||||||
| 55 | FTA card | LBCL | LN right posterior neck | Y196C | C | |
| 55.1 | Frozen/FFPE | DLBCL | Subsequent | LN right posterior neck | Y196C | C |
a
Confirmation done by direct sequencing.
b
No additional samples were available for DNA extraction for these cases.
Abbreviations: C, confirmed; DLBCL, diffuse large B-cell lymphoma; FFPE, formalin-fixed paraffin-embedded; FL, follicular lymphoma; LBCL, large B-cell lymphoma; LN, lymph node; NHL, non-Hodgkin lymphoma; NT, not tested; U-DLBCL/BL (DHL), unclassifiable, with features between diffuse large B-cell lymphoma and Burkitt lymphoma (dual hit lymphoma).