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Chronic ethanol intake and abstinence results in significantly decreased nucleus accumbens (NAc) and ventral tegmental area (VTA) Clock expression. Clock expression in the NAc (a) and VTA (b) at ZT4 and ZT16 was normalized to 18s, and fold change was calculated using the ΔΔCT method (ΔCTethanol−ΔCTcontrol). *p<0.05. ZT, zeitgeber time.
