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Mismatch repair protein IHC. (A) IHC staining shows absence of nuclear expression of MLH1 in malignant glands, while internal control lymphocytes and stromal cells have intact MLH1 immunopositivity. (B) The same tumor displays loss of PMS2 in tumor cell nuclei. (C) IHC staining for MSH2 in the same tumor shows intact nuclear expression. (D) Intact MSH6 in the same case. Original magnification, x200 (A–D).
