Figure 5.

KRAS mutation assay LODs using purified gDNA. Varying levels of G13D mutant gDNA were mixed with high wild-type background (10,000 wild-type cells) and analyzed with a KRAS G13D mutant assay and KRAS reference assay. The C_t difference between KRAS mutant and wild-type assays (dC_t) was calculated. KRAS mutation is detected when the dC_t is below the cutoff dC_t value generated with pure wild-type gDNA (green line). Samples containing four cell equivalents of mutant gDNA in a background equivalent of 10,000 wild-type cells were consistently detected.