Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Assay LODs using model CTC samples. Varying levels of KRAS G13D mutation-positive tumor cells (MDA-MB-231) were spiked into healthy donor blood and processed on the IsoFlux System. gDNA was amplified, purified, and tested for KRAS mutations. The Ct difference between KRAS mutant and wild-type assays (dCt) was calculated. Samples having as few as nine recovered tumor cells were detectable using the qPCR assay. Healthy control samples remained above the mutation detection cutoff.
