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. 2013 Oct 1;6(5):546–553. doi: 10.1593/tlo.13457

Figure 2.

Figure 2

The AKAP13-PDE8A fusion transcript is recurrent in CRC, and the genomic locus is rearranged in the SW480 cell line. (A) Twenty-four RNA sequence reads spanned the chimeric transcript breakpoint, passing from exon 1 of AKAP13 (ENST00000361243) to exon 3 of PDE8A (ENST00000310298). Dark colors indicate exons that are not part of the fusion transcript. (B) Genomic view of the rearranged locus, from the top showing annotated exons of the fused genes (exons belonging to genes located between and within PDE8A and AKAP13 were removed for clarification), relative RNA expression levels, and DNA copy numbers. The two latter are based on coverage data from high-throughput sequencing of RNA and DNA from the SW480 cell line. (C) The AKAP13-PDE8A fusion transcript was initially detected from the SW480 cell line, but nested RT-PCR demonstrated detectable levels from 17 additional colon cancer cell lines (see Table 2 for cell line identities). The specific breakpoints of the AKAP13-PDE8A fusion transcripts were verified by Sanger sequencing and shown to follow the consensus splicing sites of the fusion partner genes.