Skip to main content
. 2013 Oct 1;6(5):546–553. doi: 10.1593/tlo.13457

Figure 3.

Figure 3

The COMMD10-AP3S1 fusion transcript is recurrent in CRC, and the genomic locus is rearranged in the HCT116 cell line. (A) Twelve RNA sequence reads spanned the chimeric transcript breakpoint, passing from exon 4 of COMMD10 (ENST00000274458) to exon 4 of AP3S1 (ENST00000316788). Dark colors indicate exons that are not part of the fusion transcript. (B) Genomic view of the rearranged locus, from the top showing annotated exons of the fused genes (exons belonging to genes located between and within COMMD10 and AP3S1 were removed for clarification), relative RNA expression levels, and DNA copy numbers. The two latter are based on coverage data from high-throughput sequencing of RNA and DNA from the HCT116 cell line. (C) The COMMD10-AP3S1 fusion transcript was initially detected from the HCT116 cell line, but nested RT-PCR demonstrated detectable levels in 19 additional colon cancer cell lines (see Table 2 for cell line identities). The specific breakpoints of the COMMD10-AP3S1 fusion transcripts were verified by Sanger sequencing and shown to follow the consensus splicing sites of the fusion partner genes.