Figure 3.
Increasing valency increases cell binding of bivalent constructs. (A–C) Monomer and homodimer peptide-conjugated constructs bind recombinant FGFR4 in a similar manner on an FGFR4 binding ELISA. Huh-7 cell binding of IgG (D) and F(ab)2 (E) homodimer-conjugated constructs demonstrates a boost in cell binding affinity, whereas the Fab monomer and homodimer (F) have similar cell binding affinities. (G) Anti-idiotype capture levels of anti-FGFR4 monomer and homodimer compounds to measure multivalent interactions. Increased levels of FGFR4 binding is seen with the IgG homodimer. (H) Tumor and normal tissue uptake of homodimer peptide-conjugated IgG, F(ab)2, and Fab (*P < .05 vs monomer IgG).