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. 1973 Dec;26(6):880–883. doi: 10.1128/am.26.6.880-883.1973

Comparison of Three Culture Media for Isolation of Mycobacterium tuberculosis: a 6-Year Study

Paul I Liu 1,2, Douglas H McGregor 1,2, Isabelle Faucher 1,2, Willard L Jinks 1,2, Lawrence A Miller 1,2, Lance Green 1,2, J Grace Liu 1,2
PMCID: PMC379926  PMID: 4203334

Abstract

Among 2,648 specimens positive on culture for Mycobacterium tuberculosis over a 6-year period, 82% grew on Lowenstein-Jensen medium (LJ), 79% on American Trudeau Society (ATS), and 56% on Middlebrook 7H10 (7H10). When these commercial culture media were compared in regard to the number of acid-fast bacilli seen on the original smears, LJ cultures were found to have the highest isolation rates for each smear category, and 7H10 had the lowest rates. Comparing the media from the aspect of number of mycobacterial colonies produced, LJ and ATS had the highest average colony counts, followed by 7H10. These findings were relatively constant over the 6-year period. One possible reason for the low positive rate of 7H10 was the lack of CO2 enrichment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CUMMINGS M. M. Diagnostic methods in tuberculosis. II. Demonstration of M. tuberculosis by culture. Am J Clin Pathol. 1951 Jul;21(7):684–690. doi: 10.1093/ajcp/21.7_ts.684. [DOI] [PubMed] [Google Scholar]
  2. HUGHES D. E., MOSS E. S., HOOD M., HENSON M. Virulence of Mycobacterium tuberculosis: evaluation of a test, using neutral red indicator. Am J Clin Pathol. 1954 May;24(5):621–625. doi: 10.1093/ajcp/24.5_ts.621. [DOI] [PubMed] [Google Scholar]
  3. JEFFERIES M. B., HARDY A. V., SCHNEIDER N. J. The relative efficacy of culture media for the isolation of M. tuberculosis from clinical material. Am Rev Respir Dis. 1960 Feb;81:259–265. doi: 10.1164/arrd.1960.81.2.259. [DOI] [PubMed] [Google Scholar]
  4. KUBICA G. P., DYE W. E., COHN M. L., MIDDLEBROOK G. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am Rev Respir Dis. 1963 May;87:775–779. doi: 10.1164/arrd.1963.87.5.775. [DOI] [PubMed] [Google Scholar]
  5. KUBICA G. P., KAUFMANN A. J., DYE W. E. COMMENTS ON THE USE OF THE NEW MUCOLYTIC AGENT, N-ACETYL-L-CYSTEINE, AS A SPUTUM DIGESTANT FOR THE ISOLATION OF MYCOBACTERIA. Am Rev Respir Dis. 1964 Feb;89:284–286. doi: 10.1164/arrd.1964.89.2.284. [DOI] [PubMed] [Google Scholar]
  6. Krasnow I., Wayne L. G. Comparison of methods for tuberculosis bacteriology. Appl Microbiol. 1969 Nov;18(5):915–917. doi: 10.1128/am.18.5.915-917.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MIDDLEBROOK G., COHN M. L. Bacteriology of tuberculosis: laboratory methods. Am J Public Health Nations Health. 1958 Jul;48(7):844–853. doi: 10.2105/ajph.48.7.844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miliner R. A., Stottmeier K. D., Kubica G. P. Formaldehyde: a photothermal activated toxic substance produced in Middlebrook 7H10 medium. Am Rev Respir Dis. 1969 Apr;99(4):603–607. doi: 10.1164/arrd.1969.99.4P1.603. [DOI] [PubMed] [Google Scholar]
  9. SCHAEFER W. B., COHN M. L., MIDDLEBROOK G. The role of biotin and carbon dioxide in the cultivation of Mycobacterium tuberculosis. J Bacteriol. 1955 Jun;69(6):706–712. doi: 10.1128/jb.69.6.706-712.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. WHITCOMB F. C., FOSTER M. C., DUKES C. D. Increased carbon dioxide tension and the primary isolation of Mycobacteria. Am Rev Respir Dis. 1962 Oct;86:584–586. doi: 10.1164/arrd.1962.86.4.584. [DOI] [PubMed] [Google Scholar]

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